abstract：:The tissue distribution, molecular weight, and biochemical nature of an antigen detected by a mouse monoclonal antibody designated 283D3 and raised against rabbit visceral yolk sac endodermal cells, has been investigated. The antigen is located on the luminal side of apical tubules and large sub-apical vesicles in rab...

journal_title：Journal of reproductive immunology

authors： Meads TJ,Wild AE

更新日期：1993-04-01 00:00:00

abstract：:Seven monoclonal antibodies (MAs) generated against porcine zona pellucida glycoprotein, ZP3 (comprising both ZP3 alpha and ZP3 beta) were characterized for their specificities to ZP3 alpha (MA 7 and MA 28) or ZP3 beta (MA 1, MA 2, MA 10, MA 27 and MA 30) and their relative affinities in competitive ELISA. Among the s...

journal_title：Journal of reproductive immunology

authors： Bagavant H,Yurewicz EC,Sacco AG,Talwar GP,Gupta SK

更新日期：1993-04-01 00:00:00

abstract：:Recently developed synthetic Mimetic affinity ligands were screened for their ability to purify estradiol antibody from sheep serum. One of these ligands (color coded Yellow 1) effectively separated immunoglobulin from other serum protein by negative affinity. When 1 ml antiserum diluted 70% (protein content approxima...

journal_title：Journal of reproductive immunology

authors： Squire RS,Greenwood JV,Sanford LM

更新日期：1993-03-01 00:00:00

abstract：:Lactoferrin (LF) and transferrin (Trf) are glycoproteins with strong affinities for ferric ions. Human syncytiotrophoblastic membranes analyzed by enzyme linked immunosorbent assay (ELISA) and immunoblotting were negative with monoclonal and polyclonal antibodies to LF. Immunohistological studies of 35 normal placenta...

journal_title：Journal of reproductive immunology

authors： Thaler CJ,Labarrere CA,Hunt JS,McIntyre JA,Faulk WP

更新日期：1993-01-01 00:00:00

abstract：:Monoclonal antibodies (MAb) were raised against a testicular membrane fraction from 18-day post coitum (p.c.) rat testes. One antibody, designated 4B6.3E10 (mu, kappa), was obtained which specifically reacted with gonocytes in the fetal testis. No significant cross-reactivity with other tissues from the 18-day p.c. em...

journal_title：Journal of reproductive immunology

authors： van Dissel-Emiliani FM,van Kooten PJ,de Boer-Brouwer M,de Rooij DG,van der Donk JA

更新日期：1993-01-01 00:00:00

abstract：:Interleukin-1 alpha (IL-1 alpha) and interleukin-1 beta (IL-1 beta) were identified in samples of first trimester and term placentas by immunocytochemistry using two sets of polyclonal antibodies to recombinant IL-1. All samples contained the two species of IL-1, which predominated in different types of cells: IL-1 al...

journal_title：Journal of reproductive immunology

authors： Hu XL,Yang Y,Hunt JS

更新日期：1992-10-01 00:00:00

abstract：:The present study has demonstrated that human extravillous trophoblast cells isolated from first trimester placentae can bind granulocyte-macrophage colony-stimulating factor (GM-CSF). We have used a technique which incorporates a phycoerythrin (PE)-conjugated GM-CSF in association with a monoclonal antibody against t...

journal_title：Journal of reproductive immunology

authors： Loke YW,King A,Gardner L,Carter NP

更新日期：1992-06-01 00:00:00

abstract：:The cross reactivities of acidic extracts from boar, bovine, hamster, human and ram spermatozoa to a polyclonal antibody of the boar proacrosin binding protein has been investigated. The pH 3.0 extracts of the washed spermatozoa from each species were subjected to Western blot analysis using a polyclonal antibody deve...

journal_title：Journal of reproductive immunology

authors： Yi LS,Polakoski KL

更新日期：1992-04-01 00:00:00

abstract：:The majority of anti-sperm monoclonal antibodies which were shown to inhibit in vitro fertilization in our laboratory react with antigens in the acrosomal region of spermatozoa. To elucidate the mechanism of human and/or mouse fertilization inhibition, the effects of these antibodies on mouse sperm-zona binding and mo...

journal_title：Journal of reproductive immunology

authors： Fann CH,Lee CY

更新日期：1992-03-01 00:00:00

abstract：:It has been proposed that PGE2 is an important immunosuppressant acting at the fetal-maternal interface during pregnancy. We have previously shown that horse conceptus-conditioned medium suppresses lymphocyte proliferation. This experiment was designed to determine if horse conceptus-derived immunosuppressive activity...

journal_title：Journal of reproductive immunology

authors： Roth TL,White KL,Thompson DL Jr,Horohov DW

更新日期：1992-01-01 00:00:00

abstract：:The effects of an immunosuppressive factor (ISF) isolated from boar seminal vesicle fluid on in vitro and in vivo mouse development were investigated. It was found that the zona pellucida of porcine and mouse oocytes pre-incubated with ISF reacted in indirect immunofluorescence with antibodies against ISF. Further res...

journal_title：Journal of reproductive immunology

authors： Veselský L,Dostál J,Stanĕk R,Procházka R,Vinter P

更新日期：1991-09-01 00:00:00

abstract：:In a prospective study 140 couples who had at least three spontaneous abortions (RSA) were studied for the presence of Fc receptor blocking antibodies detected by the erythrocyte antibody rosette inhibition (EAI) assay, for anti-paternal cytotoxic antibodies (APCA), and for mixed lymphocyte culture inhibiting (MLCI) a...

journal_title：Journal of reproductive immunology

authors： Kuhn U,Blasczyk R,Hojnacki B,Vögeler U,Luboldt W,Passarge E,Fischer WM,Grosse-Wilde H

更新日期：1991-07-01 00:00:00

abstract：:We have previously shown that in patients with idiopathic premature ovarian failure there were significant changes in lymphocyte subsets. To test our hypothesis that these changes were due to oestrogen deficiency we studied lymphocyte subsets in patients with oestrogen deficiency due to other causes. Blood was taken f...

journal_title：Journal of reproductive immunology

authors： Ho PC,Tang GW,Lawton JW

更新日期：1991-05-01 00:00:00

abstract：:Sperm antigens are being sought for the development of prototype contraceptive vaccines. For vaccine-induced antisperm antibodies to inhibit the function of viable sperm, target antigens must be expressed on the sperm surface before, during or after the acrosome reaction. The purpose of this study was to develop an ap...

journal_title：Journal of reproductive immunology

authors： Fichorova R,Anderson DJ

更新日期：1991-05-01 00:00:00

abstract：:Trophoblast cells forming the reactive interface between the mother and her semiallogeneic fetus risk attack by cellular and humoral elements of the maternal immune system. Biochemical, molecular, and immunohistologic studies have identified membrane cofactor protein (MCP) and decay accelerating factor (DAF) on tropho...

journal_title：Journal of reproductive immunology

authors： Hsi BL,Hunt JS,Atkinson JP

更新日期：1991-04-01 00:00:00

abstract：:A monoclonal anti-human sperm antibody (Mab 1A1) has been produced by fusion of myeloma cells with splenocytes from a BALB/c mouse immunized with in vitro capacitated human spermatozoa. Immunofluorescence studies with Mab 1A1 show that it recognizes an antigen(s) (Ag 1A1) which is located in the acrosome of human sper...

journal_title：Journal of reproductive immunology

authors： Dubova-Mihailova M,Mollova M,Ivanova M,Kehayov I,Kyurkchiev S

更新日期：1991-04-01 00:00:00

abstract：:The immunosuppressive activity of a JEG-3 choriocarcinoma-derived factor in human IL-2-dependent T cell responses has been studied, together with its effect on IL-2-independent T cell responses induced by 10 nM TPA. The factor completely suppressed the IL-2-independent proliferative responses of T cells but failed to ...

journal_title：Journal of reproductive immunology

authors： Matsuzaki N,Saji F,Okada T,Sawai K,Kameda T,Tanizawa O

更新日期：1991-02-01 00:00:00

abstract：:Substrains of BALB/c mice differ in their susceptibility to experimental autoimmune orchitis (EAO), with BALB/cJ representative of the non-responders and BALB/cBy representative of the responders. We examined whether the susceptibility of these two substrains could be altered by reciprocal adoptive transfer of lymphoi...

journal_title：Journal of reproductive immunology

authors： Mahi-Brown CA,Tung KS

更新日期：1990-10-01 00:00:00

abstract：:The proportion of stromal cells expressing DR antigens of the HLA system or reacting with an anti-macrophage antibody was studied by immunohistochemical methods in human proliferative, secretory and post-menopausal endometria. DR+ cells showed a rounded morphology with short expansions and represented 13-25% of the st...

journal_title：Journal of reproductive immunology

authors： Laguens G,Goñi JM Jr,Laguens M,Goñi JM,Laguens R

更新日期：1990-09-01 00:00:00

abstract：:Intra-testicular transplants are placed in rodents into the large lymphatic sinusoids of the interstitial tissue of the testis. These sinusoids are filled with a protein-rich extracellular fluid that supplies all the requirements of the grafts until vascularization takes place. The testicular microvascular endothelium...

journal_title：Journal of reproductive immunology

authors： Setchell BP,Uksila J,Maddocks S,Pöllänen P

更新日期：1990-08-01 00:00:00

abstract：:The presence of a local immunosuppressive environment in the vicinity of the developing fetus has been suggested to explain survival of the semi-allogeneic fetus in a potentially hostile maternal immunologic environment. The presence of nonspecific suppressor cells in the uterus of pregnant mice has been well-document...

journal_title：Journal of reproductive immunology

authors： Kamel S,Wood GW

更新日期：1990-06-01 00:00:00

abstract：:A high resolution two dimensional electrophoretic technique (2-D), isoelectric focusing followed by polyacrylamide gel electrophoresis, for separation of neutral and acidic human spermatozoal proteins was established. Silver stained 2-D gels revealed over 260 proteins with Mr 20-200 kDa and pI between 4.5 and 7.8. Mem...

journal_title：Journal of reproductive immunology

authors： Naaby-Hansen S

更新日期：1990-06-01 00:00:00

abstract：:Purified midgestation murine trophoblast cannot be killed by a variety of cell-mediated effector mechanisms, with the exception of highly lytic effectors such as lymphokine-activated killer cells. We now report that this trophoblast population is also resistant to tumor necrosis factor (TNF)-alpha. ...

journal_title：Journal of reproductive immunology

authors： Drake BL,Head JR

更新日期：1990-03-01 00:00:00

abstract：:This work investigates the production of prostaglandin E2 (PGE2), a well known modulator for the suppression of immune cells, by pre-implantation human conceptuses from the 4-cell stage to the hatched blastocyst and by cumulus oophorus cells, these being obtained from an IVF/ET program. Cumulus cell complexes cultured...

journal_title：Journal of reproductive immunology

authors： Holmes PV,Sjögren A,Hamberger L

更新日期：1990-03-01 00:00:00

abstract：:A decrease of natural killer cell activity (NKCA) during human pregnancy might contribute to the acceptance of the allogeneic fetus by the maternal host. The inhibition of NKCA might be due to serum factors derived from the trophoblast. We focused especially on the role of immune complexes, as it has already been desc...

journal_title：Journal of reproductive immunology

authors： Struyf NJ,De Cleyn KM,Bridts CH,De Clerck LS,Buytaert PM,Stevens WJ

更新日期：1989-12-01 00:00:00

abstract：:We have previously shown that progesterone-treated lymphocytes of healthy pregnant women can produce a 34 kDa protein, progesterone-induced blocking factor (PIBF) capable of blocking lymphocyte function in vitro. Lymphocytes of women with idiopathic threatened pre-term delivery failed to produce this factor. On the as...

journal_title：Journal of reproductive immunology

authors： Szekeres-Bartho J,Varga P,Pejtsik B

更新日期：1989-09-01 00:00:00

abstract：:Prolactin (PRL) influences immune reactivity in animals and in humans and both T-cell abnormalities and reduced natural killer (NK) cell activity have been reported in women with pathological hyperprolactinemia. To investigate further the possible interactions between PRL and the immune system in humans, we analysed T...

journal_title：Journal of reproductive immunology

authors： Nicoletti I,Gerli R,Orlandi S,Migliorati G,Rambotti P,Riccardi C

更新日期：1989-05-01 00:00:00

abstract：:Female CBA/J (H-2k) mice mated with male DBA/2J (H-2d) mice show a high level of fetal resorption, which can be reduced by immunization with BALB/c (H-2d) spleen cells. The morphologically defined fetal resorption rate upon which evaluation of the outcome of pregnancy has previously been based in this strain combinati...

journal_title：Journal of reproductive immunology

authors： Heine O,Neppert J,Mueller-Eckhardt G

更新日期：1989-05-01 00:00:00

abstract：:Peripheral blood levels of K cells were measured in normal pregnant and post-partum women by a plaque-forming cell technique that detects K cells on the basis of their activity of antibody-dependent cell-mediated cytotoxicity (ADCC). Peripheral K cells decreased throughout normal pregnancy (n = 46; 7.8 +/- 3.4%, P les...

journal_title：Journal of reproductive immunology

authors： Asari S,Iwatani Y,Amino N,Tanizawa O,Miyai K

更新日期：1989-03-01 00:00:00

abstract：:The numbers of intra-epithelial lymphocytes per 5 mm of uterine epithelium were counted in cyclic (n = 18) and pregnant (n = 18) gilts on days 10, 13, 16 and 19 postestrus. In cyclic animals these cells increased (P less than 0.01) from 26.4 +/- 1.1 per 5 mm at the mid-luteal phase (day 10) to 47.7 +/- 1.5 per 5 mm at...

journal_title：Journal of reproductive immunology

authors： King GJ

更新日期：1988-10-01 00:00:00

abstract：:We have previously shown that DBA/1 mice immunized with heterologous type II collagen showed remission of the subsequent collagen-induced arthritis (CIA) when pregnant, but experienced exacerbation postpartum. Measurement of anticollagen antibody (aCa) responses by ELISA in primiparous mice immunized at day 1 of pregn...

journal_title：Journal of reproductive immunology

authors： Whyte A,Hasan Z,Rutter F

更新日期：1988-08-01 00:00:00

abstract：:Human pre-implantation stage embryos cultured in vitro spontaneously secreted a factor capable of inducing histamine-release from human blood basophils. The embryo-derived histamine-releasing factor (EHRF) has been isolated from the culture medium by means of heparin-Sepharose affinity chromatography. The factor bound...

journal_title：Journal of reproductive immunology

authors： Cocchiara R,Di Trapani G,Azzolina A,Albeggiani G,Geraci D

更新日期：1988-06-01 00:00:00

abstract：:Ten monoclonal antibodies (MAbs) raised against porcine zonae pellucidae (PZP) were checked for their reactivity against zonae of other species. Two (MAbs A6 and C10) out of six MAbs checked reacted with canine zonae as ascertained by indirect immunofluorescence. One of these cross-reactive MAbs (A6) inhibited the pen...

journal_title：Journal of reproductive immunology

authors： Bamezai AK,Mahi-Brown CA,Talwar GP

更新日期：1988-06-01 00:00:00

abstract：:Supernatants from mixed lymphocyte reactions performed in the presence of pregnancy serum (PS) or normal human serum (NHS) were tested for interleukin-1 (IL-1) activity in a thymocyte assay. A lower response was obtained with PS-containing supernatants, suggesting that PS exerts its suppressive effect by inhibiting IL...

journal_title：Journal of reproductive immunology

authors： Garcia Domingo C,Moreno A,Palomino P

更新日期：1988-06-01 00:00:00

abstract：:Several kinds of circulating autoantibodies and peripheral blood lymphocyte subsets were studied in 20 patients with secondary amenorrhea manifesting hormonal and clinical features of premature ovarian failure (POF). More than one kind of autoantibody was detected in 14 patients (70%). Seven patients (35%) had anti-th...

journal_title：Journal of reproductive immunology

authors： Miyake T,Sato Y,Takeuchi S

更新日期：1987-11-01 00:00:00

abstract：:There is conflicting evidence for the deposition of complement in spiral arteries in normal and abnormal human pregnancies. The immunogold silver staining (IGSS) technique was used to investigate the distribution of C1q, C3d, C4, C6 and C9 within the spiral arteries of formalin-fixed normal pregnancy hysterectomy spec...

journal_title：Journal of reproductive immunology

authors： Wells M,Bennett J,Bulmer JN,Jackson P,Holgate CS

更新日期：1987-10-01 00:00:00

abstract：:Effector cells associated with an aborting fetus appear to be both thymus derived (T) and natural killer (NK) cells. In order to test the hypothesis that NK cells are a major effector mediating early spontaneous abortion (less than day 8-10), CBA female mice mated by DBA/2 males were treated with either polyinosinic/c...

journal_title：Journal of reproductive immunology

authors： de Fougerolles AR,Baines MG

更新日期：1987-06-01 00:00:00

abstract：:Pregnancy-induced hypertension (PIH) can be complicated by maternal or fetal thrombocytopenia, or both. In order to investigate possible immunologic causes of these thrombocytopenias, platelet-associated IgG (PAIgG) and IgM (PAIgM) were measured in mothers with PIH and in their infants and compared with those from pat...

journal_title：Journal of reproductive immunology

authors： Rote NS,Lau RJ,Harrison MR,Branch DW,Scott JR

更新日期：1987-04-01 00:00:00

abstract：:This study examines the distribution of two carbohydrate determinants recognized by monoclonal antibodies EMA-1 and EMA-6 on cells in culture, adult mouse tissues and day 0-7 embryos, and provides partial biochemical characterization of the molecules carrying the determinants for EMA-1 on embryonal carcinoma cells. Bo...

journal_title：Journal of reproductive immunology

authors： Hahnel AC,Eddy EM

更新日期：1987-02-01 00:00:00

abstract：:Using a passive haemagglutination assay, agglutinins against trophoblast components were detected in term maternal sera at titres above those of non-pregnant control sera. These agglutinin titres were independent of the family relationship between individual sera and trophoblast samples. They were unaffected by adsorp...

journal_title：Journal of reproductive immunology

authors： Nickson DA,Sutcliffe RG

更新日期：1986-12-01 00:00:00